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random hexanucleotide primers  (Millipore)


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    Structured Review

    Millipore random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexanucleotide+primers/random+hexamer+primers/pm37371461-64-43-46
    Average 90 stars, based on 1 article reviews
    random hexanucleotide primers - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Comprehensive analysis of cis- and trans -acting factors affecting Break-Induced Replication
    Article Snippet: .. Total RNA was isolated using the hot acid-phenol method , treated with RNase-free DNase I (Qiagen) and reverse transcribed using the Superscript III reverse transcriptase (Life technologies) with random hexanucleotide primers (Sigma-Aldrich). .. Quantitative PCR amplification of cDNA was carried out using the SYBR Premix Ex Taq II (Ozyme) using the following oligonucleotides: THI5_F (5’-AAGACTACACCGCCGTCA-3’) and THI5_R (5’-AGCAAGCCAACTTGTCAATTC-3’) for THI5 ; RAD51_cDNA_F (5’-TTCTTCCACCACGCGATTAG-3’) and RAD51_cDNA_R (5’-AGATCGCGAACACACATTCA-3’) for RAD51 ; RAD52_cDNA_F (5’-CTGCTAGCTCAAACCCAGAG-3’) and RAD52_cDNA_R (5’-ACTCGCTGGAATATGCTTGG-3’) for RAD52 ; ACT1_F (5’-CTATGTTACGTCGCCTTGGA-3’) and ACT1_R (5’-TTTGGTCAATACCGGCAGAT-3’) for ACT1 used as a control.

    Reverse Transcription:

    Article Title: Comprehensive analysis of cis- and trans -acting factors affecting Break-Induced Replication
    Article Snippet: .. Total RNA was isolated using the hot acid-phenol method , treated with RNase-free DNase I (Qiagen) and reverse transcribed using the Superscript III reverse transcriptase (Life technologies) with random hexanucleotide primers (Sigma-Aldrich). .. Quantitative PCR amplification of cDNA was carried out using the SYBR Premix Ex Taq II (Ozyme) using the following oligonucleotides: THI5_F (5’-AAGACTACACCGCCGTCA-3’) and THI5_R (5’-AGCAAGCCAACTTGTCAATTC-3’) for THI5 ; RAD51_cDNA_F (5’-TTCTTCCACCACGCGATTAG-3’) and RAD51_cDNA_R (5’-AGATCGCGAACACACATTCA-3’) for RAD51 ; RAD52_cDNA_F (5’-CTGCTAGCTCAAACCCAGAG-3’) and RAD52_cDNA_R (5’-ACTCGCTGGAATATGCTTGG-3’) for RAD52 ; ACT1_F (5’-CTATGTTACGTCGCCTTGGA-3’) and ACT1_R (5’-TTTGGTCAATACCGGCAGAT-3’) for ACT1 used as a control.

    Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter.
    Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM random hexanucleotide primers (SigmaAldrich). qPCR mRNA analysis was performed on an Mx3000PTM Quantitative PCR System Thermocycler (Stratagene, San Diego, MA, USA) using SYBR-green fluorescence quantification technology (KAPA SYBR FAST qPCR Kit, Kapa Biosystems, Wilmington, MA, USA). ..

    Article Title: Eroded telomeres are rearranged in quiescent fission yeast cells through duplications of subtelomeric sequences
    Article Snippet: .. Total RNA was treated three times with RNase-free DNase I (Qiagen) and reverse transcribed using the Superscript III reverse transcriptase (Life technologies) with random hexanucleotide primers (Sigma-Aldrich). ..

    Article Title: The Mycobacterium tuberculosis phoPR Operon Is Positively Autoregulated in the Virulent Strain H37Rv
    Article Snippet: .. One microgram of RNA was mixed with 25 pmol of random hexanucleotide primers (Sigma) and 50 units of Expand reverse transcriptase (Roche) in a final volume of 20 μl. ..

    Article Title: Oxytocin can regulate myometrial smooth muscle excitability by inhibiting the Na + ‐activated K + channel, Slo2.1
    Article Snippet: .. The Qiagen RNeasy Kit (catalogue no. 74124; Qiagen, Valencia, CA, USA) was used to isolate total RNA from primary hMSMCs, uterine tissue obtained from pregnant and non‐pregnant patients, and hTERT‐HM cells. cDNAs from these RNA preparations and from total human brain RNA (Ambion catalogue no. AM7962; Thermo Fisher Scientific Inc., Waltham, MA, USA) were generated with Superscript III Reverse Transcriptase (catalogue no. 18080‐044; Invitrogen, Carlsbad, CA, USA) and random hexanucleotide primers from Sigma‐Aldrich (catalogue no. H0268‐1UN; Sigma‐Aldrich, St Louis, MO, USA). .. The Qiagen RNeasy Kit (catalogue no. 74124; Qiagen, Valencia, CA, USA) was used to isolate total RNA from primary hMSMCs, uterine tissue obtained from pregnant and non‐pregnant patients, and hTERT‐HM cells.

    Article Title: An in vitro model for investigating human autologous neuronal-astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy.
    Article Snippet: cells (a) expressed morphological and cytoskeletal markers of differentiated neurons (MAP2a&b; Tau) or astrocytes (GFAP) in anticipated proportion (1:2), and (b) regenerated synaptic connections and neural–astrocytic associations.. Co-cultures with autologous blood leukocytes established that alterations in the viability (by Annexin V/PI) of brain and immune cells over 3 days were indicative of neurodegenerative or immunosuppressive processes.. During co-culture, B-cells (CD19þ) remained largely unaffected while T-lymphocytes (CD3þ) and monocytes (CD14þ) declined, consistent with immunosuppressive process.

    Article Title: RPA and Pif1 cooperate to remove G-rich structures at both leading and lagging strand
    Article Snippet: .. Total RNA was treated with RNase-free DNase I (Qiagen) and reverse transcribed using the SuperscriptIII reverse transcriptase (Life technologies) with random hexanucleotide primers (Sigma-aldrich). .. Quantitative PCR amplification of cDNA was carried out using the SYBR Premix Ex Taq II (Ozyme) with these cycling parameters: 1 cycle at 95°C for 30 sec, followed by 45 cycles at 95°C for 10 sec, 58°C for 15 sec, and 72°C for 20 sec, and a final extension step at 72°C for 5 min. PIF1 cDNA was quantified by using the oligonucleotides Pif1-forward (5′-CTGAAAACTCATTTGACCAG-3′) and Pif1-reverse (5′-GCAATCTTTTCTCCAAATTGC-3′).

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular responses of Prunus avium (wild cherry) embryonic axes to temperatures affecting dormancy.
    Article Snippet: • Seed dormancy and dormancy cycling are poorly understood at the molecular level, but are central to plant community development.. This study focuses on the embryonic axes of deeply dormant seeds of Prunus avium (wild cherry).. • Rapid amplification of cDNA ends (RACE), differential display and quantitative PCR were used to recover and monitor expression of cDNAs.

    Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter.
    Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM random hexanucleotide primers (SigmaAldrich). qPCR mRNA analysis was performed on an Mx3000PTM Quantitative PCR System Thermocycler (Stratagene, San Diego, MA, USA) using SYBR-green fluorescence quantification technology (KAPA SYBR FAST qPCR Kit, Kapa Biosystems, Wilmington, MA, USA). ..

    cDNA Synthesis:

    Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter.
    Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM random hexanucleotide primers (SigmaAldrich). qPCR mRNA analysis was performed on an Mx3000PTM Quantitative PCR System Thermocycler (Stratagene, San Diego, MA, USA) using SYBR-green fluorescence quantification technology (KAPA SYBR FAST qPCR Kit, Kapa Biosystems, Wilmington, MA, USA). ..

    Article Title: An in vitro model for investigating human autologous neuronal-astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy.
    Article Snippet: cells (a) expressed morphological and cytoskeletal markers of differentiated neurons (MAP2a&b; Tau) or astrocytes (GFAP) in anticipated proportion (1:2), and (b) regenerated synaptic connections and neural–astrocytic associations.. Co-cultures with autologous blood leukocytes established that alterations in the viability (by Annexin V/PI) of brain and immune cells over 3 days were indicative of neurodegenerative or immunosuppressive processes.. During co-culture, B-cells (CD19þ) remained largely unaffected while T-lymphocytes (CD3þ) and monocytes (CD14þ) declined, consistent with immunosuppressive process.

    Fluorescence:

    Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter.
    Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM random hexanucleotide primers (SigmaAldrich). qPCR mRNA analysis was performed on an Mx3000PTM Quantitative PCR System Thermocycler (Stratagene, San Diego, MA, USA) using SYBR-green fluorescence quantification technology (KAPA SYBR FAST qPCR Kit, Kapa Biosystems, Wilmington, MA, USA). ..

    Generated:

    Article Title: Oxytocin can regulate myometrial smooth muscle excitability by inhibiting the Na + ‐activated K + channel, Slo2.1
    Article Snippet: .. The Qiagen RNeasy Kit (catalogue no. 74124; Qiagen, Valencia, CA, USA) was used to isolate total RNA from primary hMSMCs, uterine tissue obtained from pregnant and non‐pregnant patients, and hTERT‐HM cells. cDNAs from these RNA preparations and from total human brain RNA (Ambion catalogue no. AM7962; Thermo Fisher Scientific Inc., Waltham, MA, USA) were generated with Superscript III Reverse Transcriptase (catalogue no. 18080‐044; Invitrogen, Carlsbad, CA, USA) and random hexanucleotide primers from Sigma‐Aldrich (catalogue no. H0268‐1UN; Sigma‐Aldrich, St Louis, MO, USA). .. The Qiagen RNeasy Kit (catalogue no. 74124; Qiagen, Valencia, CA, USA) was used to isolate total RNA from primary hMSMCs, uterine tissue obtained from pregnant and non‐pregnant patients, and hTERT‐HM cells.

    Purification:

    Article Title: An in vitro model for investigating human autologous neuronal-astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy.
    Article Snippet: cells (a) expressed morphological and cytoskeletal markers of differentiated neurons (MAP2a&b; Tau) or astrocytes (GFAP) in anticipated proportion (1:2), and (b) regenerated synaptic connections and neural–astrocytic associations.. Co-cultures with autologous blood leukocytes established that alterations in the viability (by Annexin V/PI) of brain and immune cells over 3 days were indicative of neurodegenerative or immunosuppressive processes.. During co-culture, B-cells (CD19þ) remained largely unaffected while T-lymphocytes (CD3þ) and monocytes (CD14þ) declined, consistent with immunosuppressive process.



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